Coding

Part:BBa_J70519

Designed by: Julie Norville   Group: Knight Lab   (2009-12-08)


formed by cutting J70423 with PsrI and adding annealed J70483 and J70484

R0010.J70514 formed by cutting J70423 with PsrI and adding annealed J70483 and J70484 (R0010.(J70482.E0040 scarless))

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 886


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Categories
Parameters
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